Mesenchymal stromal cells (MSCs) have recently attracted increasing attention as an ideal cell source for tissue regeneration and cell-based therapies due to ease of isolation and their significant differentiation capacity. Despite their excellent properties, it has suggested to be further characterized when extracted from different sources before any biomedical applications. In present study, we compared human bone marrow (BM), adipose tissue (AT), breast, and umbilical cord blood-derived MSCs (UCB-MSCs) for surface antigen expression and differentiation ability. MSCs were isolated from human abdominal, breast, BM, UCB tissues. Flow cytometric analysis was done to analyze the surface antigens of isolated MSCs. Cultured MSCs were compared in terms of surface marker expression, differentiation capabilities, and both fibroblast growth factor (FGF) and receptor expression. MSCs derived from abdominal, breast, BM and UCB tissues exhibited similar expression patterns of surface markers and fibroblast-like morphology. MSCs from breast and abdominal tissues had greater proliferative potential than bone marrow-derived MSCs. UCB-MSCs had the highest rate of cell proliferation. All MSC subgroups showed overexpression of the anti-inflammatory surface marker CD206. UCB-MSCs exhibited more potent immunomodulatory effects than BM-MSCs and adipose-derived MSCs. A significant difference was found in expression of interferon-γ, basic FGF, hepatocyte growth factor, insulin-like growth factor-1, and stem cell-derived factor-1 between different MSCs subgroups. Although the data indicate some similarities between MSCs derived from abdominal and breast tissues, UCB-MSCs exhibited higher angiogenesis in comparison to other sources, making UCB-MSCs a useful model for clinical applications of cell therapy such as wound healing.