Do you get overwhelmed designing high-dimensional full-spectrum flow cytometry panels? Have you ever designed a panel on paper and not been able to get the panel to work at the cytometer? Do you need to analyse data from multiple timepoints but have issues with batch to batch variation? Join us for an interactive workshop covering all aspects of theoretical full-spectrum panel design and practical panel optimisation. This workshop will cover all steps required to go from a set of markers on paper to having data ready for use in high-dimensional data analysis algorithms. We’ll follow a case study to demonstrate common mistakes and interactively find solutions so attendees can leave the workshop better equipped to follow this process for their own research.